Synovial Fluid Analysis and Culture, Extended Incubation

CPT Code(s):
87070, 87075, 83872, 89060, 89051, 87205, 87101

Turnaround Time:
48 hours

Specimen Requirements

Preferred Specimen:
Synovial Fluid

Minimum Volume:
1.0 mL

Transport Container:
Capped Syringe (without the needle) or Sterile Cup and Lavender Top Tube

Transport Temperature:
Room Temperature

Collection Instructions:
Follow normal aseptic collection proceudres

Rejection Criteria:
Quantity not sufficient for analysis; Specimen received at room temperature > 2 hours old; Specimen received refrigerated > 48 hours old; frozen specimens

Stability Criteria

Room Temperature:
24 hours

Refrigerated:
7 days

Frozen:
Unacceptable

Clinical Significance

The Synovial Fluid Analysis and Culture, Extended Incubation is an important diagnostic tool used to assess the synovial fluid found in joints. It has specific clinical significance and diagnostic use in the following ways:

1. Detection of Slow-Growing Pathogens: The extended incubation period allows for the growth of slow-growing pathogens that may not be detected in a standard 48-hour culture. Some infectious agents, such as Mycobacterium tuberculosis or fungal pathogens, require an extended incubation period to grow and be identified. This test can aid in the diagnosis of infections caused by these slower-growing pathogens.

2. Identification of Unusual Pathogens: In certain cases, the cause of joint infection or inflammation may not be readily apparent. The extended incubation period allows for the detection and identification of unusual or rare pathogens that may be causing the joint symptoms. This can help guide appropriate treatment strategies.

3. Assessment of Antibiotic Resistance: The extended incubation period also allows for the detection of antibiotic-resistant organisms that may not be evident in a shorter incubation period. This information is crucial in guiding the selection of appropriate antibiotics for the treatment of joint infections.

4. Evaluation of Persistent or Recurrent Joint Infections: In cases of persistent or recurrent joint infections, the extended incubation test can help identify the underlying cause and guide further management. It can determine if there is a chronic infection that requires prolonged or different treatment approaches.

5. Guiding Treatment Decisions: The results of the extended incubation test can provide valuable information for guiding treatment decisions. It can help healthcare providers select appropriate antimicrobial therapy tailored to the specific pathogens identified. This information is essential for effective management of joint infections.

In summary, the Synovial Fluid Analysis and Culture, Extended Incubation is a valuable diagnostic tool for assessing joint infections, particularly those caused by slow-growing or unusual pathogens. It aids in identifying antibiotic-resistant organisms, evaluating persistent or recurrent joint infections, and guiding treatment decisions. This test plays a crucial role in ensuring accurate diagnosis and appropriate management of joint-related conditions.